Webtaining 2% thiourea, and electrophoresed (CHEF Mapper XA system; Bio-Rad). The resultant patterns were analyzed, and a dendrogram was produced (BioNumerics v.6.6; Applied Maths, Sint-Martens-Latem, Belgium) using a 2% Dice correlation and optimization coefficient. Isolates that shared >90% identity were considered as a pulsotype. WebOct 17, 2024 · To remove fragmented DNA background in tumor samples via electrodepletion, plugs were loaded into a 1% certified low-melt agarose gel in 0.5× TAE buffer and run in the CHEF Mapper XA System at 14 ...
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WebNote, however, that the CHEF Mapper and Pippin Pulse systems require at least 50 ng of DNA for analysis and are recommended only if there is sufficient DNA sample available. DNA Sizing QC Method Comments Procedure ... Agilent Technologies, Inc. Bio-Rad ® CHEF Mapper ® XA Pulsed Field Electrophoresis System Requires >50 ng Procedure ... WebNov 1, 2024 · PFGE was used to verify the linear plasmid carrying the large inserts and quality of genomic segments with 1% agarose gel in 0.5 × TBE using the CHEF Mapper XA System with fixed parameters (6 V cm −1, 1–25 s, 16–18 h, 120°) and buffer circulation at 14 °C. After PFGE, the gel was stained with ethidium bromide and the DNA bands were ... birchy brae
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WebCHEF Mapper ® XA Pulsed Field Electrophoresis System Instruction Manual and Application Guide Catalog Numbers 170-3670 to 170-3673 For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) WebCHEF Genomic DNA Plug Kits; CHEF Mapper XA System 2; CHEF-DR III Variable Angle System; CHEF-DR II System 2; Chelex 100 Molecular Biology Grade Resin; Chelex 100 Resin; Chromatographic Surfaces 2; Chromatography Standards 2; ChromLab Software 3; Clarity Western ECL Substrate; CM Affi-Gel Blue Gel; WebPulseNet PFGE protocols have been developed and validated for use on Bio-Rad CHEF Mapper XA System or CHEF-DR III System. A list of equipment and supplies needed … dallas tx 10 day weather forecast