WebMay 15, 2024 · CytoFLEX Daily Startup, QC and Shutdown Workflow Overview Daily Startup procedure for CytoFLEX To be followed by the first user of the day (weekdays … WebApr 11, 2024 · The cells were resuspended in FACS buffer (PBS containing 1% FCS, 1 mM EDTA, and 0.05% NaN3) supplemented with 0.2 μg/ml propidium iodide (PI) to exclude dead cells.

CytoFLEX ‒ FCCF ‐ EPFL

WebBoth can be used as a total DNA stains and exclusion dyes > (many people use PI as an exclusion dye to examine cell membrane integrity > and judge cell viability). > > Upon fixation, the cell membrane integrity is compromised hence both SYTO9 > and PI become total DNA stains. WebJul 8, 2012 · Step 2: Using the DN region from Step 1, gate a FS versus SS plot on these DN events, and draw a tight region around the subset of cells with low FS, now called Debris. Step 3: Invert this Debris gate, creating a Not-Debris gate, and use this Not-Debris gate on the total population for your downstream Annexin V vs. PI display. cisco anyconnect エラー 一覧

Facility: UC Flow Cytometry Core Research Directory

WebA guide to gating in flow cytometry. Flow cytometry analysis typically begins with creating gates to distinguish cells of interest. This process of gating can appear quite random to a flow cytometry novice but it is in fact the most important part of flow cytometry analysis. So what gating methods do you need to know to confidently analyze your ... WebLaser: 405 nm: violet: Filters: 450/45: Brilliant Violet (BV) 421, Pacific Blue, BD Horizon 450 : 525/40: BV510, AmCyan, BD Horizon V500 : 610/20: VBV605, Qdot 605 WebApr 6, 2024 · For apoptosis assay, the Annexin V-FITC/PI Apoptosis Detection Kit (Telenbiotech, China) was used. Briefly, cells were cultured with FBS-free culture medium. After 24 h starvation, cells were collected and stained with FITC and PI for 15 min at room temperature. The apoptotic cells were measured by flow cytometry (Cytoflex, Beckman). cisco anyconnect インストール手順