Optiphase supermix

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WebJan 16, 2024 · Although botulinum neurotoxin serotype A (BoNT/A) products are common treatments for various disorders, there is only one commercial BoNT/B product, whose low potency, likely stemming from low affinity toward its human receptor synaptotagmin 2 (hSyt2), has limited its therapeutic usefulness. WebSep 1, 2016 · RNA binding YSi SPA beads and OptiPhase SuperMix scintillation cocktail were purchased from PerkinElmer. UniverSol-ES liquid scintillation cocktail was purchased from MP Biomedicals (Santa Ana, CA). Bovine serum albumin (BSA) fraction V, fatty acid-free, was purchased from Roche (Basel, Switzerland).

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WebAfter incubation, the amounts of 14 CO 2 captured in the filters were monitored by the addition of scintillation fluid (e.g., OptiPhase Supermix or MicroScint™ O; 50 μl/well). Radioactivity was counted in a scintillation counter for multiwell plates (e.g., MicroBeta® Trilux or TopCount from Perkin-Elmer). how many calories in toasted ravioli

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Webacid and 2.5 ml liquid scintillation cocktail (OptiPhase SuperMix; Perkin Elmer, Boston, MA, USA) and counted on a liquid scintillation counter (Microbeta Trilux; Perkin Elmer). Results are reported as the ratio of radioactivity in the brain to that in the perfusate (R br, μl/g): R br ¼ C brain C perfusate ð1Þ where C WebNov 15, 2013 · To analyze the influence of saponins on the cell membrane-cholesterol we developed a cell culture model using ECV-304 urinary bladder carcinoma cells and … WebAt the end of the incubation time, either 400 μL of the medium was added to counting tubes with UniverSol-ES liquid scintillation cocktail or 90 µL of the medium was added to a flexiplate with OptiPhase SuperMix cocktail (24- and 96-well plates, respectively) to detect either [3 H] or [14 C], respectively, which corresponds to AA release. The ... high risk account monitoring procedure

Cell-based multiwell assays for the detection of substrate accumulation …

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Web0.15 ml Optiphase “SuperMix” scintillation mixture. Instrument efficiency was 40%. Perfusion data were plotted as counts per minute versus fraction number. Fractions col-lected before and after the peak were used to calculate baseline as a single exponential de-cay. The calculated baseline was subtracted from the experimental data ... WebAug 20, 2016 · The medium (100 μl) was then transferred to PET 24 well sample counting plates containing 300 μl OptiPhase SuperMix scintillation cocktail for counting. Fresh medium (0.5 ml) was applied to the cells, and then removed after 0 , 0.5, 1, 2, 5, 15, 60 and 1440 min of exposure. 100 μl aliquots of the fresh medium were transferred to ... high risk accommodation responseWebJan 29, 1999 · The bound IP was eluted with 1.0 M ammonium formate in 0.1 M formic acid. Fifty microliters of the eluate from each well was transferred into a Wallac 96-well plate and mixed with 200 μl of Optiphase supermix. The plate was covered with a plastic plate seal and the contents were mixed by shaking on a microtiter plate shaker for 10 min. how many calories in tomato puree

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Optiphase supermix

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WebJan 31, 2024 · OptiPhase SuperMix ® liquid scintillation cocktail (purchased from PerkinElmer Life Sciences, Waltham, MA). 10. 30% glacial acetic acid. 11. Pierce Bradford … WebOptiPhase Supermix liquid scintillation cocktail (50 µl per well) are pipetted into a rigid 96-well plate and the 1 µl samples are added, with one empty well between samples to avoid …

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Webylamide (LSD), [3H]-melatonin, and OptiPhase SuperMix in the assay were purchased from Perkin-Elmer Life Sciences (NEN). Human MT 1 /MT 2 receptor membrane proteins were also Perkin-Elmer FIGURE 1 Chemical structure of agomelatine (A), structure of the series of derivatives of agomelatine (B), and GW117 (C) WebJan 29, 1999 · The bound IP was eluted with 1.0 M ammonium formate in 0.1 M formic acid. Fifty microliters of the eluate from each well was transferred into a Wallac 96-well plate …

WebTrade name: OptiPhase SuperMix (Contd. of page 2) 41.0 CAS: 9016-45-9 NLP: 500-024-6 Alkylphenol Polyglycolether Aquatic Chronic 2, H411 Skin Irrit. 2, H315; Eye Irrit. 2, H319 … WebScintillation Cocktail Solution part number OPTIPHASESUPERMIXCOCKTAIL Scintillation cocktail liquid optiphase supermix 5 l NSN 6640-01-541-0381. 321-802-5889 321-733 …

WebSep 9, 2010 · After 4 hours of incubation, the plates were centrifuged again at 300 g for 5 minutes and 100-μL aliquots of the supernatants from each well were transferred to a new plate containing 100 μL/well of Optiphase Supermix scintillation fluid (Perkin Elmer). Radioactivity was measured using 1450 Microbeta counter (Wallac). WebJul 27, 2011 · Optiphase “SuperMix” scintillation fluid was purchased from PerkinElmer Life Sciences. α-Conotoxin MII (α-CtxMII) was obtained from Dr. J. M. McIntosh (University of Utah, Salt Lake City, UT). All other chemicals were reagent grade and, unless specified otherwise, were purchased from Sigma-Aldrich, including ascorbic acid, atropine ...

WebWelcome to Optiphase, Inc. online center; a leading provider of interferometric fiber optic sensor solutions On July 16, 2013, Optiphase sold its assets to Halliburton Energy …

WebSep 18, 2007 · After the effector cell addition, the cells were incubated 12–16 h before being harvested, when 25 μl of cell supernatant was added to 175 μl of OptiPhase SuperMix scintillation fluid (Wallac, Loughborough, Leicestershire, U.K.) and analyzed with a MicroBeta reader (PerkinElmer, Wellesley, MA). high risk acquirersWebMay 27, 2016 · The Optiphase™ supermix and Hionic-Fluor™ liquid scintillation cocktails and Solvable™ were purchased from Perkin Elmer (Coventry, UK). Liposomal amphotericin B (AmBisome®, Gilead Sciences, Foster City, CA, USA) and amphotericin B deoxycholate (Fungizone®, ER Squibb, Uxbridge, UK) were reconstituted according to manufacturer's … how many calories in tomato slicesWebPerkinElmer liquid scintillation fluid optiphase supermix solution. Liquid Scintillation Fluid Optiphase Supermix Solution, supplied by PerkinElmer, used in various techniques. Bioz … high risk act in prisonWebOptiphase Supermix scintillation cocktail (2.5mL) was added to each sample. Triplicate samples of 100mL aliquots of the perfusion medium were treated in the same manner as … how many calories in tomato basil soupWebOptiPhase Supermix and UniFilterH-96 GF/B were delivered by PerkinElmer (Shelton, CT, US). MitoTrackerHRed FM, Bodipy 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene) and Hoechst 33258 were obtained from Molecular Probes, Invitrogen (Carlsbad, CA, US). Nitrocellulose membrane was from Amer- high risk activities bc wildfireWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health … how many calories in toasted breadWebOptiphase HiSafe 3, had been selected as a result of a previous comparative study (BLG-632, September 1991). 1.1 Experimental setup In a first stage, a limited market study was performed to establish the range and variety of different, commercially available, liquid scintillation cocktails available for purchase. how many calories in tinned salmon